Ractopamine (Rac) Rapid Test Strip (Colloidal Gold) food safety Diagnostics equipment
Ractopamine (Rac) (2 ppb) Feed Rapid Test Strip
Ractopamine (Rac) Rapid Test Strip (Colloidal Gold)
Store at 2-30℃ in dry environment.
Expiry date: 12 months
The test strip is used for detecting Ractopamine in feed.
The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Rac in samples, it will combine with marked antibodies, prevent the marked antibodies from combining the Ractopamine antigens of NC nitrocellulose membrane.
If Rac contents in sample solution are greater than detection limit, the test line will not display color reaction and the result is positive; If Rac contents in sample solution are less than detection limit, the test line will display purple and the result is negative.
Kit sensitivity: 2 ppb (ng/ml)
(Final detection limit = Kit sensitivity × dilution times of the sample)
Test strip ………………………… 50T/kit
【Materials required But Not Supplied】
Equipments: mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, and balance with a reciprocal sensibility of 0.01g
Micropipettes: single-channel 20 to 200µl and 100 to1000µl
Reagents: methanol, N-hexane, anhydrous sodium sulfate.
Lab gown must be clean and use disposable pipette to avoid contamination that interfere results.
Sample pretreatment step:
Weight 1.0 ± 0.05g crushed homogeneous feed samples, add 1g anhydrous sodium sulfate, then add 10ml methanol, shake well for 30s, and centrifuge at room temperature at 4000 r/min for 10 min
Take 1ml supernatant into a glass tube and blow dry at 50 -60℃ with nitrogen or air.
Add 1ml deionized water to dissolve the dry residues, then add 1ml N-hexane, shake well for 30s, and centrifuge at room temperature at speed of 4000 r/min for 5min.
Use 80 L of the lower aqueous layer for the assay.
Dilution times of the sample: 10 detection Limits: 20ppb
Tear test card foil pouch, remove the test card, put on a flat, clean work surface
Use straw draw the ready sample, add 2-3 drop (about 80ul) sample into the sample hole.
Please determine the result within 5-10min.
Negative Positive Invalid
Negative: control line(C) and test line (T) are both seen purple red
Positive: only control line(C) is seen purple red;
Invalid: control line (C) isn’t seen purple red.
Don’t use expired or damaged products.
The test card removed from refrigerator should place at room temperature and should be used as soon as possible to avoid failure when wet.
Don’t touch sample hole and white nitrocellulose (NC) membrane.
Don’t mix micropipette to avoid cross contamination
Samples should be clear, not turbid particles and not clogged; if not, it will easily lead to blocking and inconspicuous color, which will affect the judgment of the experimental results.