|Nature:||Colloidal Gold||Keep Temperature:||２－8℃|
|Packaging Material:||Aluminum Foil||Package Dimensions:||12ｘ７ｃｍ|
fish test kit,
food bacteria testing kit
NiroFuracilin metabolite colloidal gold method rapid detection kit Product antibiotic residue test strips
NiroFuracilin metabolite colloidal gold method rapid detection kit
This product is used to rapidly detect nitrofuracilin metabolites (SEM) residues in muscle tissues of livestock, poultry and aquatic products. The whole testing process is about 60 minutes, which is suitable for all kinds of enterprises and testing institutions. The SEM detection limit of this product is 0.5 ppb (ug/kg).
Specifications: 10 times / box;
|Extract Sol. 1||Derivatization reagent 1 bottle|
|Extract Sol. 2||Extract Sol. 3|
|Purifier 1||NitroFuracilin metabolite complex solution 1 bottle|
|10 Nitrofuracilin rapid test cards (including micropores)|
[Improved but required equipment]
Homogenizer, balance, centrifuge, nitrogen (air) gas blow dryer, vortex mixer, pipette, water bath.
Muscle tissue samples (shrimp should be cleaned after removing the head and shell, and the fish should be washed after removing the scales). If it is not detected in time, it should be stored in the dark (within 24 hours).
1. Take a certain amount of de-fat muscle tissue sample and mash it with a homogenizer;
2. Weigh 3g of homogenized sample in a 20ml centrifuge tube, add 200uL of derivatization reagent, then add 4ml of extractant 1 and shake for 2 minutes;
Incubate for 30 min at 3.75 °C water bath;
4. After taking out, accurately add 1 ml of extractant 2, then add 5 ml of extractant 3, shake and mix for 3 min, 4000 rpm, and centrifuge for 5 min;
5. Pipette 3 ml of the supernatant into a 5 ml centrifuge tube, and dry at 75 ° C with a nitrogen (air) air blow dryer.
6. Add 1 ml of scavenger to the blown centrifuge tube, oscillate for 30 s, then add 500 uL of nitrofuran metabolite-specific complex solution, mix well for 30 s, 4000 rpm, centrifuge for 5 min (or let stand to Obviously layered);
7. The lower layer solution is the sample liquid to be tested;
Before testing, restore the test card and the test solution to room temperature (20 ° C ~ 30 ° C). Remove the test card from the original bag and place it horizontally on the front of the observer, as shown below;
Pipette the test solution 100uL into the gold standard micropore, gently blow for 30s, dissolve the red substance in the hole, and wait for the reaction for 5 minutes;
All the solution in the suction hole is added to the sample hole, and the time starts after the sample is applied; the result should be read in 6~10 minutes, and the other time is invalid.
Note: The pretreatment of the four samples of furan is completely uniform, and one sample can simultaneously detect four drug residues. The sample to be tested can also be used for chloramphenicol residue detection.
Negative (-): T-line color development (detection line, near the end of the sample well) is deeper or deeper than the C line (control line), indicating that the concentration of the drug to be tested in the sample is below the detection limit or contains no drug to be tested.
Positive (+): The color of the T line is significantly lighter than the C line, indicating that the concentration of the drug to be tested in the sample is higher than the detection limit. The shallower the T line than the C line, the higher the concentration of the drug to be tested in the sample.
Invalid: The C line does not appear, indicating that the incorrect operation process or the test card has failed. Read the instructions carefully and retest with the new test card. 【Precautions】??
The nitrofuran metabolites and their derivatives are highly susceptible to degradation, and the test should be completed quickly according to the instructions;
The detection process must be carried out in an environment of 20 ° C ~ 30 ° C; try not to touch the white film surface in the center of the reagent plate; wear gloves when testing;
Do not use the reagents provided; do not reuse the supplied dropper to avoid cross-contamination;
|Kind||Cross-reaction rate (%)|
[Storage conditions and expiration date]
Store at 4 ° C ~ 30 ° C protected from light, do not freeze; this product is valid for 12 months.
[Production date and batch number]
See the bag for details.