|Category:||Test Card||Nature:||Quick Test Card|
diagnosis of animal diseases,
rabies diagnosis in dogs
Newcastle disease antibody rapid test card
Newcastle disease, also known as Asian chicken mites, is an acute high-level infectious disease of chickens and a variety of birds caused by viruses. The main features are dyspnea, diarrhea, neurological disorders, mucosal and serosal bleeding. Due to the different pathogenic strains, they can be manifested as diseases with large differences in severity.
The Newcastle Disease Virus (NDV) antigen rapid detection card uses a rapid immunochromatographic assay to detect Newcastle disease virus antigens. After the sample to be tested is added to the well, it is moved along the chromatographic membrane along with the colloidal gold-labeled anti-NDV monoclonal antibody. If an NDV antigen is present in the sample, it binds to the antibody on the test line to display wine red. If the NDV antigen is not present in the sample, no color reaction occurs.
1 quick test card 20 sets
2 sterile cotton swabs 20
3 dropper 20
4 sample buffer 20 bottles
5 disposable sanitary gloves 1 pack
6 instructions 1 copy
Method of operation
1. Use a cotton swab to collect samples from the cloaca (recommended) or the trachea. The cotton swab should be inserted into the cloaca or trachea and repeatedly rubbed on the inner wall. For young birds, fresh faeces can also be taken directly from cotton swabs.
2. Immediately insert the cotton swab into the test tube containing the sample buffer, rotate the cotton swab repeatedly on the test tube wall at least 10 times, and mix the solution to dissolve the sample as much as possible in the solution. Squeeze the cotton swab on the wall of the test tube above the liquid level to allow the liquid to be squeezed out as much as possible and discard the cotton swab.
3. If the particles in the stool are large, allow to stand for 1-2 minutes to precipitate large particles.
4. Remove the test card from the sealed bag. Place the test card on a flat surface, use a pipette to draw the upper clear liquid from the test tube, and slowly and drop 5 drops to the well with the “S” mark.
5. Leave the test at room temperature for 10-15 minutes. If the result is more than 15 minutes, the result is invalid.
Positive: In the observation hole, the detection line area (T) and the control line area (C) simultaneously appear magenta lines (Fig. 1).
Negative: In the observation well, only a reddish line appears in the control line (C) (Fig. 2).
Failure: In the observation hole, no color line appears in the control line area (C) and the detection line area (T); or only the detection line area (T) appears color line (Fig. 3, 4).
Storage method : Store at 2 - 30 o C.
Validity: 24 months.
1. Products damaged in aluminum foil bags cannot be used. The test card must be used within 1 hour after removal.
2. Samples should be tested immediately to avoid long-term storage.
3. The collection of samples directly affects the accuracy of the test, so ensure that the sample is collected correctly and adequately.
4. Excessive collection of stool samples can affect the normal surge of liquid and lead to erroneous results (black bands appear), while too few samples may have false negative results, so please control the sample size according to the diagram.
5. Foam should be avoided when adding samples to the test card well.
6. Used test cards should be disposed of in accordance with local pollutant disposal regulations.
7. Products that have expired cannot be used.
8. The used test cards and samples must be disposed of as pollutants according to relevant local regulations.
1. What are the limitations of this product?
This reagent is a qualitative screening reagent that can detect Newcastle disease virus antigens with extreme precision. However, if the sample is insufficient or the antigen concentration is lower than the sensitivity of the reagent test, a negative result may occur. The doctor should make a diagnosis after considering all the clinical symptoms. An accurate clinical diagnosis should not be based on a single test result.
2. What is the sensitivity and specificity of this product?
Compared with other similar rapid test cards, the test card detected Newcastle disease virus antigen sensitivity of > 99%, and there was no cross-reaction with avian cholera, infectious bronchitis and avian influenza with similar symptoms.
3. Is there a positive reaction when the vaccination is performed?
If an attenuated vaccine is inoculated, the virus will increase in the body during the immunization process and may show a positive reaction. However, this phenomenon automatically disappears after 3–10 days of vaccination.