Store at 2-8℃ in dry environment.
commingled cow milk above or below tolerance and/or safe levels.
The kit is a competitive colloidal gold immunoassay method. Sample is added
(BLQ) antigens of NC nitrocellulose membrane.
【Technique Data】
Kit sensitivity: 4 ppb (ng/ml) (Final detection limit)
【Detection Limits】
β-Lactam
MRL
(μg/L)
Results
(μg/L) Cephalosporin
MRL
(μg/L)
Results
(μg/L)
Penicillin G 4 2-4 Cefquinome 20 15-20
Ampicillin 4 3-4 Cefacetrile 125 100
Amoxicillin 4 4-5 Cefalonium 20 18-20
Oxacillin 30 6-8 Cefazolin 50 40-50
Cloxacillin 30 6-8 Cefoperazone 60 50-60
Dicloxacillin 30 6-8 Cephapirin -- 80
Nafcillin 30 20-30 Ceftiofur 100 90-100
【Kit Contents
Test strip ………………………… 12*8T/kit
96 well tray and sample well with reagent.
Instruction …………………………..1
【Milk Sample Preparation】
1. Collect milk sample from butch, or farm
2. Make sure the milk has no clot.
3. Milk at room temperature, don’t heat or freeze.
4. Thoroughly mix with the reagent before testing.
【Test Procedure】
1. Read the manual instruction before testing. Be sure the raw
commingled milk and test kit is at room temperature.
2. Take one Tube from the box, open tube and take out decided
number of test to be perform, Use the test kit within an hour beyond
time may cause failure of the test. The unused test strip must be sealed
and keep out from sunlight and prevent moisture.
3. Take the milk sample, drop 200ul (about 4-5drops) into the sample
well, and thoroughly dissolve the milk with reagents for 5 minutes till
the milk presents pink color.
4. Insert the test strip into the sample-well, read the results after 5
minutes.
【Results Judgment】
Negative(-): control line(C) display red, test line(T)
color is deeper than the control line or the same as
the C line, the result shows that (BLQ) concentration
is less than detection Limits or not containing (BLQ).
Positive(+): control line(C) display red, test line(T)
color is shallower than the control line or no color,
the result shows that (BLQ) concentration is higher
than detection Limits.
Invalid: control line (C) doesn’t display, it means the
operational process is incorrect or this test card has
lost efficacy.
【Notice】
Don’t use the expired or damaged products
The test card removed from refrigerator
should place at room temperature and
should be used as soon as possible to avoid
failure when wet
Don’t touch sample hole and white
nitrocellulose (NC) membrane
Don’t mix micropipette to avoid cross
contamination
Samples should be clear, not turbid particles
and not clogged; if not, it will easily lead to
blocking and inconspicuous color, which will
affect the judgment of the experimental
results.
