Aflatoxin B1 (AFB1) Rapid Test Strip (Colloidal Gold) Colloidal gold rapid test kit
MTUSBIO® Aflatoxin B1
(AFB1) 3ppb Rapid Test Strip (Colloidal Gold)
【Product Name】
Aflatoxin B1 (AFB1) Rapid Test Strip (Colloidal Gold)
【Specification】 50T/kit
【Storage Conditions】
Store at [2-30℃ in dry environment.
Expiry date: 12 months
【Intended Application】
The test strip is used for detecting Aflatoxin B1 in cereal and feed.
【Principle】
The kit is using competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Aflatoxin B1 in samples, it will combine with marked antibodies, prevent the marked antibodies from combining the Aflatoxin B1 antigens of NC nitrocellulose membrane.
If Aflatoxin B1 contents in sample solution are greater than detection limit, the test line will not display color reaction and the result is positive; If Aflatoxin B1 contents in sample solution are less than detection limit, the test line will display purple and the result is negative.
【Technique Data】
Kit sensitivity: 3 ppb (ng/ml)
(Final detection limit = Kit sensitivity × dilution times of the sample)
Detection Limits
Cereal, Feed………………….……..15ppb
【Kit Content】
Test strip ………………………… 50T/kit
Instruction …………………………..1
【Materials required But Not Supplied】
Equipments: mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, and balance with a reciprocal sensibility of 0.01g
Micropipettes: single-channel 20 to 200µl and 100 to1000µl
Reagents: methanol, N-hexane.
【Sample Pre-treatment】
Instructions
Lab gown must be clean and use disposable pipette to avoid contamination that interfere results.
Solution preparation before sample pre-treatment
Liquor 1: Sample extracts solution:
70% Methanol solution, Methanol (V): Distilled water (V) =7:3
Sample pretreatment step:
1. Cereal, Feed:
Weight 2g crushed homogeneous samples into a 15ml centrifuge tube, add 2ml Sample extract solution (Liquor 1), oscillate for 5min fully, centrifuge at room temperature at 4000 r/min for 10 min. (if some samples have strong water absorption, it may lead to the failure of sampling, we can be appropriately increase dosage of sample extract solution, last, dilution times, in terms of actual amount add sample extracts solution)
Take 0.1ml supernatant into a tube; add 0.4ml distilled water, mix.
Use 80 L for the assay.
