Cle-Rac-Sal Rapid Test Strip food drug residue test kit
(Colloidal GoldCle-Rac-Sal Rapid Test Strip)
【Product Name】
Cle-Rac-Sal Rapid Test Strip (Colloidal Gold)
【Specification】 50T/kit
【Storage Conditions】
Store at [2-30℃] in dry environment.
Expiry date: 12 months
【Intended Application】
The test strip is used for detecting Clenbuterol (Cle), Ractopamine (Rac), and Salbutamol (Sal) in feed.
【Principle】
The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is residues in samples, it will combine with marked antibodies, prevent the marked antibodies from combining the residue antigens of NC nitrocellulose membrane.
If residues contents in sample solution are greater than detection limit, the test line will not display color reaction and the result is positive; if residues contents in sample solution are less than detection limit, the test line will display purple and the result is negative.
【Technique Data】
Kit sensitivity: CLE 3 ppb/RAC 3ppb/ SAL 5ppb (ng/ml)
(Final detection limit = Kit sensitivity × dilution times of the sample)
Detection Limits (CLE / RAC / SAL )
Feed ………………….……..30ppb / 30ppb / 50ppb
【Kit Content】
Test strip ………………………… 50T/kit
Instruction …………………………..1
【Materials required But Not Supplied】
Equipments: mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, and balance with a reciprocal sensibility of 0.01g
Micropipettes: single-channel 20 to 200µl and 100 to1000µl
Reagents: methanol, N-hexane, anhydrous sodium sulfate.
【Sample Pre-treatment】
Instructions
Lab gown must be clean and use disposable pipette to avoid contamination that interfere results
Sample pretreatment step:
1. Feed:
Weight 1.0 ± 0.05g crushed homogeneous feed samples and put into 50ml centrifuge tube, add 1g anhydrous sodium sulfate, then add 10ml methanol, oscillate for 3min fully, centrifuge at room temperature at 4000 r/min for 10 min
Take 1ml supernatant into a glass tube and blow dry at 50 -60℃ with nitrogen or air dry.
Add 1ml deionized water to dissolve the dry residues, and then add 1ml N-hexane, shake well for 30s, and centrifuge at room temperature at 4000 r/min for 5min.
Use 80 L of the lower aqueous layer for the assay
Dilution times of the sample: 10
Detection Limits: CLE 30 ppb/RAC 30ppb/ SAL 50ppb
【Test Methods】
Tear test card foil pouch, remove the test card, put on a flat, clean work surface
Use straw draw the ready sample, add 2-3 drop (about 80ul) sample into the sample hole.
Please determine the result within 5-10min.
【Results Judgment】
Negative Positive Invaild Invaild
Negative: control line(C) and test line (T) are both seen purple red
Positive: only control line(C) is seen purple red;
Invalid: control line (C) isn’t seen purple red.
【Notice】
Don’t use the expired or damaged products
The test card removed from refrigerator should place at room temperature and should be used as soon as possible to avoid failure when wet
Don’t touch sample hole and white nitrocellulose (NC) membrane
Don’t mix micropipette to avoid cross contamination
Samples should be clear, not turbid particles and not clogged; if not, it will easily lead to blocking and inconspicuous color, which will affect the judgment of the experimental results
