Deoxynivalenol (DON) Rapid Test Strip (Colloidal Gold) Improve Product Safety Quality
Deoxynivalenol (DON) Rapid Test Strip
Deoxynivalenol (DON) Rapid Test Strip (Colloidal Gold)
Store at 2-30℃ in dry environment.
Expiry date: 12 months
The test strip is used for detecting Deoxynivalenol in Cereal and feed.
The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Deoxynivalenol in samples, it will combine with marked antibodies, preventing the marked antibodies from combining the Deoxynivalenol antigens of (NC) nitrocellulose membrane.
If Deoxynivalenol is in sample solution are greater than detection limit, the test line will not display therefore color reaction and the result is positive; If Deoxynivalenol is in sample solution but less than the detection limit, the test line will display purple and the result is negative.
Kit sensitivity: 20 ppb (ng/ml)
(Final detection limit = Kit sensitivity × dilution times of the sample)
Cereal; Feed ………………….……..100ppb
Test strip ………………………… 50T/kit
【Materials required But Not Supplied】
Equipment: mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, and balance with a reciprocal sensibility of 0.01g
Micropipettes: single-channel 20 to 200µl and 100 to1000µl
Reagents: methanol, N-hexane.
Lab gown must be clean and use disposable pipette to avoid contamination that interfere results.
Solution preparation before sample pre-treatment
Liquor 1: Sample extracts solution: 70% Methanol solution, Methanol (V): Deionized water (V) =7:3.
Sample pretreatment step:
1. Cereal, Feed:
Weight 1g crushed homogeneous samples into a 50ml centrifuge tube, add 5ml deionized water, oscillate for 5min fully, centrifuge at room temperature at 4000 r/min for 10 min.
Use 80 L supernatant for the assay.
Dilution times of the sample: 5 detection Limits: 100ppb
Tear test card foil pouch, remove the test card, put on a flat, clean work surface
Use pipette to withdraw the ready sample, and slowly add 3 drop wisely (about 80ul) sample into the sample hole.
Please determine the result within 8-10min.
Negative Positive Invalid
Negative: control line(C) and test line (T) are both seen purple red
Positive: only control line(C) is seen purple red;
Invalid: control line (C) isn’t seen purple red.
Don’t use expired or damaged products.
The test card removed from refrigerator should place at room temperature and should be used as soon as possible to avoid failure when wet.
Don’t touch sample hole and white nitrocellulose (NC) membrane.
Don’t mix micropipette to avoid cross contamination
Samples should be clear, not turbid particles and not clogged; if not, it will easily lead to blocking and inconspicuous color, which will affect the judgment of the experimental results.