Zearalenone (ZEN) Rapid Test Strip (Colloidal Gold) food drug residue food drug residue test kit
Zearalenone (ZEN) (30ppb) Cereal; Feed Rapid Test Strip (Colloidal Gold)
【Product Name】
Zearalenone (ZEN) Rapid Test Strip (Colloidal Gold)
【Specification】 50T/kit
【Storage Conditions】
Store at 2-30℃ in dry environment.
Expiry date: 12 months
【Intended Application】
The test strip is used for detecting Zearalenone in cereal and feed.
【Principle】
The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Zearalenone in samples, it will combine with marked antibodies, prevent the marked antibodies from combining the Zearalenone antigens of NC nitrocellulose membrane.
If ZEN contents in sample solution are greater than detection limit, the test line will not display color reaction and the result is positive; If ZEN contents in sample solution are less than detection limit, the test line will display purple and the result is negative.
【Technique Data】
Kit sensitivity: 30 ppb (ng/ml)
(Final detection limit = Kit sensitivity × dilution times of the sample)
Detection Limits
Cereal; Feed ………………….……..30ppb
【Kit Content】
Test strip ………………………… 50T/kit
Instruction …………………………..1
【Materials required But Not Supplied】
Equipment’s: mixer or stomacher, oscillator, centrifuge, measuring pipets, and balance with a reciprocal sensibility of 0.01g
Micropipettes: single-channel 20 to 200µl and 100 to1000µl
Reagents: acetonitrile, N-hexane.
【Sample Pre-treatment】
Instructions
Lab gown must be clean and use disposable pipette to avoid contamination that interfere results.
Solution preparation before sample pre-treatment
Liquor 1: Sample extracts solution:
90% Acetonitrile, acetonitrile (V): Deionized water (V) =9:1
Sample pretreatment step:
1. Cereal; Feed:
Weight 2g crushed homogeneous samples into a 15ml centrifuge tube, add 2ml Sample extract solution (Liquor 1), oscillate for 5min fully, centrifuge at room temperature at 4000 r/min for 10 min. (if some samples have strong water absorption, it may lead to the failure of sampling, we can be appropriately increase dosage of sample extract solution, last, dilution times, in terms of actual amount add sample extracts solution)
Take 0.1ml supernatant into a tube, add 0.9ml deionized water, and mix for standby.
Dilution times of the sample: 10 detection Limits: 100ppb
【Test Methods】
Tear test card foil pouch, remove the test card, put on a flat, clean work surface
Use micropipette to withdraw the ready sample, add 3 drop (about 80ul) sample into the sample hole
Please determine the result within 8-10min.
【Results Judgment】
Negative Positive Invalid
Negative: control line(C) and test line (T) are both seen purple red
Positive: only control line(C) is seen purple red;
Invalid: control line (C) isn’t seen purple red.
【Notice】
Don’t use the expired or damaged products
The test card removed from refrigerator should place at room temperature and should be used as soon as possible to avoid failure when wet
Don’t touch sample hole and white nitrocellulose (NC) membrane
Don’t mix micropipette to avoid cross contamination
Samples should be clear, not turbid particles and not clogged; if not, it will easily lead to blocking and inconspicuous color, which will affect the judgment of the experimental results
