|Category:||Colloidal Gold||Nature:||Quick Test Card|
|Keep Temperature:||２－8℃||Package Dimensions:||12ｘ７ｃｍ|
rabies diagnosis in dogs,
antibody rapid test card
Animal disease diagnosis Pet dog Canine distemper antigen rapid detection card
Canine distemper is often prevalent in a variety of carnivores, especially in dogs that are more susceptible to infection through the respiratory system. Because the disease is difficult to cure, the mortality rate is high. The dog can detect the virus in the blood after infection with canine distemper virus (CDV) for about 1 week. After about 2 weeks of infection, fever, dry cough, conjunctivitis, vomiting, diarrhea and other symptoms appear. The canine distemper antigen rapid detection card uses a rapid immunochromatographic detection technique for detecting canine distemper virus antigens. After the sample is added to the well, it is moved along the chromatographic membrane along with the colloidal gold-labeled anti-CDV monoclonal antibody. If a CDV antigen is present in the sample, it binds to the antibody on the test line to display wine red. If the CDV antigen is not present in the sample, no color reaction occurs.
1 quick test card 20 sets
2 sterile cotton swabs 20
3 dropper 20
4 sample buffer 20 bottles
5 disposable sanitary gloves 1 pack
6 instructions 1 copy
Method of operation
l Soak the cotton swab with saline solution, collect the sample from the conjunctiva or freshly discharged urine, immediately insert the cotton swab into the test tube containing the sample buffer, rotate the cotton swab repeatedly on the test tube wall at least 10 times, and mix. Solution to dissolve the specimen as much as possible in the solution. Squeeze the cotton swab on the wall of the test tube above the liquid level to allow the liquid to be squeezed out as much as possible and discard the cotton swab.
l Or collect serum or plasma, use the supplied dropper to drop two drops of sample serum and plasma into the sample buffer and mix well.
2. Remove the test card from the sealed bag. Place the test card on a flat surface, pipette the liquid from the test tube, and slowly and droply drop 5 drops into the well with the "S" mark.
3. Leave at room temperature for 10-15 minutes to judge the result. The result of more than 15 minutes is invalid.
1. Positive: In the observation hole, the detection line area (T) and the control line area (C) simultaneously have a purple-red line (Fig. 1).
2. Negative: In the observation hole, only a purple-red line appears in the control line area (C) (Fig. 2).
3. Failure: In the observation hole, no color line appears in the control line area (C) and the detection line area (T); or only the detection line area (T) appears color line (Fig. 3, 4).
Storage method : Store at 2 - 30 o C.
Validity: 24 months.
1. Products damaged in aluminum foil bags cannot be used. The test card must be used within 1 hour after removal.
2. Samples should be tested immediately to avoid long-term storage.
3. The collection of samples directly affects the accuracy of the test, so ensure that the sample is collected correctly and adequately.
4. Foam should be avoided when adding samples to the test card well.
5. Products that have expired cannot be used.
6. The used test cards and samples must be disposed of as pollutants according to relevant local regulations.
1. Which sample is used well?
In the early stages of infection with canine distemper, it is better to take blood as a sample. There are no symptoms of conjunctivitis, dry cough, diarrhea, etc. at the beginning of the infection. The virus only exists in the blood. If it is suspected that there is a symptom of canine distemper, it is convenient to use conjunctival epithelial cells and urine.
2. What should you pay attention to when testing with plasma?
When preparing plasma, if a container containing the anticoagulant EDTA is used, it may interfere with the test results. EDTA can also interfere with low concentrations, so avoid using EDTA.
3. What are the limitations of this product?
This reagent is a qualitative screening reagent that can detect canine distemper virus antigens with great precision. However, if the sample is insufficient or the antigen concentration is lower than the sensitivity of the reagent detection, a negative result may occur. The doctor should make a diagnosis after considering all the clinical symptoms. . An accurate clinical diagnosis should not be based on a single test result.
4. What is the sensitivity and specificity of this product?
Compared with other similar rapid test cards, the test card detected the antigenic sensitivity of canine distemper virus was >99%, and there was no cross-reactivity with coronavirus, adenovirus and parvovirus with similar symptoms.
5. Is there a positive reaction when the vaccination is performed?
Since the canine distemper vaccine is usually an attenuated vaccine, the virus temporarily increases in the body during the immunization process and may show a positive reaction. However, this phenomenon automatically disappears after 3–10 days of vaccination. However, animals treated with monoclonal antibodies may have false positives for a long time because they produce secondary antibodies against monoclonal antibodies.