Animal Disease Detection Card / Swine Fever Antibody Rapid Test Card

swine fever antibody rapid test card Animal disease rapid test card

Swine fever antibody rapid test card instruction

Generic name: swine fever antibody rapid test card

English name: Rapid Anti-CSFV Test

Pinyin: Zhuwen Kangti JianceKa

[Use of swine fever antibody test card]

It is used to detect Classical Swine Fever (CSF) antibodies in pig serum/plasma/whole blood samples.

[Swine fever antibody test card experimental principle]

The swine fever antibody test card uses a colloidal gold immunochromatographic technique to detect a swine fever antibody in a sample (serum, plasma or whole blood). Gold-labeled inactivated swine fever antigen (Au-Agl) was pre-coated on glass fiber paper, and hog cholera antigen (Ag2) and rabbit anti-swine antibody were coated on the nitrocellulose membrane on the detection line and the control line, respectively. When the test sample is positive, the hog cholera antibody in the sample combines with the colloidal gold-labeled hog sputum antigen (Au-Ag1) to form a complex, which moves forward along the strip due to the chromatography, passes through the test line and is pre-coated. The swine fever antigen (Ag2) forms an "Au-Ag1-swine antibody-Ag2-solid phase material" immune complex and agglomerates, and the free gold-labeled antigen is bound to the rabbit anti-swine antibody at the control line. Color development. Negative samples were only developed at the control line. The operation is simple and fast, the result is intuitive, accurate, high sensitivity and easy to judge.

[Swine fever antibody test card composition]

1. Swine fever antibody test card 50 copies

2. Instructions 1 copy

3. One dropper 50 pieces

[Swine fever antibody test card operation method]

1. Using a 1.5 ml sample tube, 0.5-1 ml of blood to be collected is naturally precipitated or centrifuged for 10 minutes or so to precipitate the serum.

2. Place the swine fever antibody test card on the table, use the pipette to absorb the test serum, and add 2 drops of about 80~100ul into the oval sample hole of the swine fever antibody test card. The result was determined by reacting at room temperature for 20 minutes. (If the swine fever antibody test card is stored at 4 ° C, it must be returned to room temperature before testing)

[The result of detection of swine fever antibody test card]

Positive: A purple-red line appears in each of the control line area (C) and the test line area (T). The darker the color of the test line (T), the higher the titer of the hog cholera antibody.

Negative: Only a purple-red line appears in the control line area (C).

Invalid: no purple red line appears or purple line appears only in the detection area (T), and no purple line appears in the control line area (C).

[Swine fever antibody test card diagnosis reference]

The reference sample can be compared with the ribbon titer of the control card 20 minutes after the sample is loaded. 1. When the color of the strip of the test sample (T) is ≥1:32 in the control card, it indicates that the titer of the swine fever antibody in the test sample is high, and the immunization of the swine fever vaccine is not needed. 2. When the color of the strip of the test sample (T) is <1:32 titer in the control card, it means that the titer of the antibody to the swine fever antibody in the test sample can not resist the minimum protective titer of the virulent attack of the swine fever. Vaccination time. 3. When there is no obvious ribbon at the test sample line (T), it indicates that there is no hog antibody in the sample to be tested. If the herd is healthy, porcine vaccination should be carried out in a timely manner.

[Swine fever antibody test card storage and expiration date]

The swine fever antibody test card should be stored at 2-30 ° C, sealed and dried for 18 months.

[Swine fever antibody detection card product features]

1, convenient: visual judgment, no equipment, simple operation;

2, fast: 5-20 minutes can produce results, which is conducive to the rapid identification of the epidemic and immune level monitoring;

3. Uses: It is suitable for extensive use at all levels of anti-inspection departments, pig farms and veterinary stations.