High Lingmin Food Safety Quick Detection Card / Estradiol Quick Test Strip

Estradiol

Quick Test Strip Instruction Manual

【specification】

8 pieces / tube

[Product introduction]

This product is suitable for the qualitative detection of estradiol in milk (including pure milk and raw milk) and goat milk. The whole detection process takes only 9 minutes, the operation is simple, and the sensitivity is high.

[Sample detection limit]

1-2ppb (ug / kg)

[Detection principle]

The estradiol rapid detection test strip applies the principle of competitive inhibition immunochromatography. During the flow of estradiol in the sample, it binds to a colloidal gold-labeled specific estradiol monoclonal antibody, which inhibits the antibody and the NC membrane. The binding of the estradiol-BSA conjugate on the detection line causes a change in the color of the detection line. When the sample does not contain estradiol or the concentration is lower than the detection limit, the T line is stronger than the C line. When the estradiol concentration in the sample is equal to or higher than the detection limit, the T-line color development is significantly weaker than the C-line color development or the T-line color is not developed or there is no significant difference between the T-line and C-line colors. Regardless of whether or not the sample contains estradiol, the quality control line C line will show color to show that the test is effective.

【product composition】

1. Test strip 8 strips / tube

2. Red dry powder micropore 8 holes / tube

3. Desiccant 2 pcs / cylinder

4.Instruction manual

[Test steps]

1. Please read the instructions carefully before use. Before use, return the test strip and sample to room temperature.

2. Remove the reagent cartridge, open the required number of microwells and test strips, and mark them. Please use it as soon as possible within 1h. Immediately after the detection reagent is removed, cover the reagent cartridge to prevent it from getting wet.

3. Using a micropipette, pipette 200 μl of the sample to be tested into the microwell, and slowly suck 5 times until the mixture is uniform, and observe no solids with the naked eye (this step is very important).

4. After incubating at 40 ± 2 ℃ for 3 minutes, insert the marked test strip into the microwell (the end of the MAX line printed down, so that it is fully immersed in the solution).

5. Immerse the test strip in the microwell for 6 minutes to interpret the results according to the schematic diagram. The interpretation at other times is invalid.

[Result Judgment]

T-line and C-line color contrast

1.T line> C line, negative, estradiol in the sample is lower than the detection limit

2. T line = C line, weak positive, estradiol in the sample is equal to the detection limit

3. T line <C line or T line is not color, positive, estradiol in the sample is higher than the detection limit

4. C line is not color, invalid, test strip is invalid, retest

[Result determination diagram]

【Precautions】

1. Test strips are disposable at room temperature; do not use expired test strips.

2. Immediately after the detection reagent is removed, cover the reagent cartridge cap. If you can't use up to 8 microwells at a time, immediately cover the remaining microwells with the microwell caps, and put them back in the reagent cartridge to keep them sealed.

3. During use, try not to touch the white film surface in the center of the test strip; avoid direct sunlight or direct fan blowing.

4. The milk sample must be a homogeneous liquid without lumps, sourness and precipitation.

5. If a positive result appears, it is recommended to repeat the test.

6. If you encounter any problems during the test, please contact the supplier.

7. The test results of this product are for reference only. For confirmation, please refer to the relevant national standard methods.

[Specificity]

Detection of 500 ppb of tetracycline, quinolone, sulfa drugs and other drugs, the results were negative.

[Storage conditions and validity]

1. Store at 2 ～ 8 ℃ in a sealed, dry and dark place; do not freeze.

2. Validity period is 12 months.