Phenylethanolamine A ColloidalGold Rapid Detection Card

Phenylethanolamine A ColloidalGold Rapid Detection Card

operation instructions

(Colloidal gold method)

1.Principle and Application

This product is made by applying the principle of competitive inhibition colloidal gold immunochromatography to detect Phenethyl alcohol A (PHEA) in urine and water samples.

After the sample solution is dropped into the sample adding hole of the detection card, the Phenethyl alcohol amine A in the sample solution is combined with the gold labeled antibody, thus preventing the gold labeled antibody from combining with the Phenethyl alcohol amine A conjugate on the cellulose membrane. When the content of Phenethyl alcohol amine A in the sample solution is greater than the detection limit, the detection line does not show color, and the result is positive; When the content of Phenethyl alcohol A in the sample solution is less than the detection limit, the detection line will show purple red, and the result is negative.

2. Technical indicators

Lower limit of sample detection

Urine sample: 3 ppb

Water Sample 20 ppb

3. Kit composition

Detection card: 50 pieces/box

Instructions: 1 copy

4. Required equipment and reagents

4.1 Instrument: Centrifuge

4.2 Micro Pipette: single channel 20 µ l-200 µ l

5. Sample pretreatment

5.1 Instructions before sample processing:

The experimental equipment must be clean and use a disposable suction head to avoid contamination and interference with the experimental results.

5.2 Pre processing steps for urine samples:

Take a clear urine sample for supernatant testing. If the urine sample is cloudy, it needs to be centrifuged at 4000r/min for 10 minutes, and the supernatant should be taken for detection.

5.3 Steps for pre-treatment of water samples:

Take a clear water sample. If there is turbidity in the water sample, centrifuge at 4000r/min for 10 minutes. Take the upper layer of clear liquid and mix it with purified water 1:3 and shake well, and wait for testing.

6. Experimental Steps

6.1 Remove the inspection card and place it on a flat and clean surface.

6.2 Use a matching pipette to absorb the prepared sample liquid, and slowly and dropwise add 2-3 drops (approximately 60ul) into the sampling hole (S).

6.3 Leave at room temperature for 8-10 minutes to determine the results. Results exceeding 10 minutes can only be used as a reference

7. Result judgment

Negative positive invalid invalid

Negative: Both the C and T lines show color, indicating that the concentration in the sample is below the detection limit or does not contain.

Positive: The C line shows red, while the T line does not show color, indicating that the concentration in the sample is above the detection limit.

Invalid: The absence of a quality control C-line indicates an incorrect operating process or a failure of the detection card.

8. Precautions

8.1 Products that have expired or have damaged aluminum foil bags cannot be used.

8.2 When removing the detection card from the refrigerator, it should be restored to room temperature before opening. The opened detection card should be used as soon as possible to avoid failure due to moisture.

8.3 Do not touch the white film surface in the center of the detection card.

8.4 Droppers should not be mixed to avoid cross contamination.

8.5 The sample solution to be tested should be clear, free from turbid particles, and bacterial contamination, otherwise it may lead to abnormal phenomena such as blockage and unclear color development, which may affect the judgment of experimental results.

9. Storage and shelf life

Storage conditions: The reagent kit should be stored in a dry environment at 2-30 ℃.

Shelf life: The product has a validity period of 1 year, and the production date can be found in the packaging box.