CPL Colloidal Gold Rapid Detection Card

CPL Colloidal Gold Rapid Detection Card

operation instructions

(Colloidal gold method)

1. Principle and Application

This product is made by applying the principle of competitive inhibition colloidal gold immunochromatography to detect CPL in tissue samples.

After the sample solution is dropped into the sample adding hole of the detection card, the CPL in the sample solution will combine with the gold labeled antibody, thus preventing the gold labeled antibody from combining with the CPL conjugate on the cellulose membrane. When the CPLcontent in the sample solution is greater than the detection limit, the detection line does not develop color (or the color is lighter than the control line), and the result is positive; When the content of CPL the sample solution is less than the detection limit, the detection line appears purplish red (the color is consistent with or deeper than the control line), and the result is negative.

2. Technical indicators

Sample detection limit:

Organization: 0.1ppb

3. Kit composition

Detection card: 40 pieces/box

Extractant A 2 bottles

Dissolving agent 2 bottles

Sample duplicate solution: 1 bottle

Instructions: 1 copy

4. Equipment and reagents that need to be provided by oneself

4.1 Instruments: homogenizer, nitrogen drying device, oscillator, centrifuge, graduated pipette, balance (sensitivity 0.01g)

4.2 Micro Pipette: single channel 20 µ l-200 µ l, 100 µ l-1000 µ l

5. Sample pretreatment

Instructions before sample processing:

The experimental equipment must be clean and use a disposable suction head to avoid contamination and interference with the experimental results.

5.1 Steps for pre-treatment of tissue samples from aquatic products, livestock, and poultry:

1) Remove the skin and fat from the samples of fish, shrimp, crab, animal meat, and viscera. Homogenize the samples with a homogenizer, weigh 2g of the samples and place them in a 15ml centrifuge tube. 2) Add 2ml of purified water (or deionized water) and 2ml of extractant A in sequence, shake well, and centrifuge at room temperature (20-25 ℃) at 4000r/min for 5 minutes;

3) Take 1ml of the upper organic phase and place it in a 1.5ml centrifuge tube. Dry it in a water bath at 50-60 ℃ using nitrogen gas (or an electric hair dryer) to obtain solid residue;

4) Add 2ml of solvent first, shake and mix evenly; Add another 0.3ml of sample solution, mix well, and let stand for about 5 minutes. The liquid is clearly stratified. Take the lower layer of liquid for backup.

6. Experimental Steps

6.1 Tear open the aluminum foil packaging bag of the inspection card, take out the inspection card, and place it on a flat and clean tabletop.

6.2 Suck the prepared sample liquid with a matching pipette, and slowly drop 2-3 drops (about 60ul) into the sample hole (S) one by one (foam should be avoided).

6.3 Leave at room temperature for 8-10 minutes to determine the results, and results exceeding 10 minutes can only be used as a reference.

7. Result judgment

Negative positive invalid

Negative: In the detection window, a purple red line appears on the control line (C), and the color of the detection line (T) is consistent or deeper than the control line.

Positive: In the detection window, the control line (C) shows a purple red line, while the detection line (T) does not show color or is lighter than the control line.

Invalid: In the detection window, there is no purple red line on the control line (C).

8. Precautions

8.1 Products that have expired or have damaged aluminum foil bags cannot be used.

8.2 When removing the detection card from the refrigerator, it should be restored to room temperature before opening. The opened detection card should be used as soon as possible to avoid failure due to moisture.

8.3 Do not touch the white film surface in the center of the detection card.

8.4 Droppers should not be mixed to avoid cross contamination.

8.5 The sample solution to be tested should be clear, free from turbid particles, and bacterial contamination, otherwise it may lead to abnormal phenomena such as blockage and unclear color development, which may affect the judgment of experimental results.

9. Safety instructions

Extractant A and solvent are flammable reagents and should be used away from ignition sources. In case of skin contact, wash thoroughly with soap and clear water. In case of eye contact, lift the eyelid, wash with clear water or normal saline, and seek medical advice. In case of accidental ingestion, drink enough warm water to induce vomiting, or use 1:5000 Potassium permanganate or 5% Sodium thiosulfate solution to wash the stomach, and seek medical advice.

10. Storage and shelf life

Storage conditions: The reagent kit should be stored in a dry environment at 2-30 ℃.

Shelf life: The product has a validity period of 1 year, and the production date can be found in the packaging box.