Furazolidone Colloidal Gold RapidDetection Card

Furazolidone Colloidal Gold RapidDetection Card

operation instructions

(Colloidal gold method)

1. Principle and Application

This product is made by applying the principle of competitive inhibition colloidal gold immunochromatography to detect Furazolidone metabolites (AOZ) in egg samples.

After the sample solution is dropped into the sample adding hole of the detection card, the Furazolidone metabolites in the sample solution combine with the gold labeled antibody, thus preventing the gold labeled antibody from combining with the Furazolidone conjugate on the cellulose membrane. When the content of Furazolidone metabolites in the sample solution is greater than the detection limit, the detection line does not show color, and the result is positive; When the content of Furazolidone metabolites in the sample solution is less than the detection limit, the detection line will show purple red, and the result is negative.

2. Technical indicators

Lower limit of sample detection:

Eggs...... 0.5ppb

3. Kit composition

Detection card: 40 pieces/box

Reagent 1: 1 bottle

Reagent 2: 1 bottle

Reagent 3: 1 bottle

Solvent 1: 1 bottle

Solvent 2: 1 bottle

Extractant A: 4 bottles

Dissolving agent: 1 bottle

Sample duplicate solution: 1 bottle

Derivatization reagents 2 bottles

Instructions: 1 copy

4. Required equipment and reagents

4.1 Instruments: homogenizer, nitrogen drying device, oscillator, centrifuge, graduated pipette, balance (sensitivity 0.01g)

4.2 Micro Pipette: single channel 20 µ l-200 µ l, 100 µ l-1000 µ l

4.3 Reagent: None

5. Sample pretreatment

5.1 Instructions before sample processing:

The experimental equipment must be clean and use a disposable suction head to avoid contamination and interference with the experimental results.

5.2 Egg Sample Processing Method

1) Use a homogenizer to homogenize samples (egg yolk or whole egg) at low speed; Weigh 2.0 ± 0.05g of homogenized egg samples into a 10ml centrifuge tube, add 4ml of deionized water, 0.5ml of reagent 1 solution, and 200 µ l of solvent 1, and shake with an oscillator for 1 minute to mix well;

2) Add 200 µ l of solvent 2, shake thoroughly with an oscillator for 5 minutes, over 3000g, and centrifuge for 10 minutes; Take all the supernatant into a 15ml centrifuge tube, add 600 µ l derivative reagent, and shake thoroughly with an oscillator,

3) Incubate in a 65 ℃ water bath for 30 minutes;

4) Add 1ml of reagent 2 solution, 0.4ml of reagent 3 solution, and 5ml of extractant A respectively, and shake for 5 minutes;

5) 4000 rpm at room temperature, centrifuge for 5 minutes;

6) Take 2.5ml of the upper liquid into another centrifuge tube and dry it with nitrogen or air at 50-60 ℃;

7) Dissolve the residue with 1ml of solvent, add 0.25ml of sample complex solution, shake thoroughly and mix for 30 seconds; Room temperature 4000 rpm, centrifuge for 5 minutes;

8) The upper layer is a solvent, and the lower sample solution is taken for analysis.

6. Experimental steps

6.1 Tear open the aluminum foil packaging bag of the inspection card, take out the inspection card, and place it on a flat and clean tabletop.

6.2 Suck the prepared sample liquid with a matching pipette, and slowly drop 2-3 drops (about 60ul) into the sample hole (S) one by one (foam should be avoided).

6.3 Leave at room temperature for 8-10 minutes to determine the results. Results exceeding 10 minutes can only be used as a reference.

7. Result judgment

Negative positive invalid

Negative (-): both lines C and T are red, indicating that the concentration of Furazolidone in the sample is lower than the lower limit of detection, or does not contain.

Positive (+): If the C line shows red and the T line does not show color, it means that the concentration of Furazolidone in the sample is higher than the lower limit of detection.

Invalid: The absence of a quality control C-line indicates an incorrect operating process or a failure of the detection card.

8. Precautions

8.1 Products that have expired or have damaged aluminum foil bags cannot be used.

8.2 When removing the detection card from the refrigerator, it should be restored to room temperature before opening. The opened detection card should be used as soon as possible to avoid failure due to moisture.

8.3 Do not touch the white film surface in the center of the detection card.

8.4 Droppers should not be mixed to avoid cross contamination.

8.5 The sample solution to be tested should be clear, free from turbid particles, and bacterial contamination, otherwise it may lead to abnormal phenomena such as blockage and unclear color development, which may affect the judgment of experimental results.

9. Storage and shelf life

Storage conditions: The reagent kit should be stored in a dry environment at 2-30 ℃.

Shelf life: The product has a validity period of 1 year, and the production date can be found in the packaging box.