Aflatoxin M1 fast test card operation instructions

Aflatoxin M1 (AFM1-0.5)

Rapid Test Kit

【Product Name】

Aflatoxin M1 (AFM1-0.5) Rapid Test Strip

【Specification】 96T/kit

【Storage Conditions】

• Store at 2-8℃ in dry environment.
• Expiry date: 12 months

【Intended Application】

The test strip is used for detecting Aflatoxin M1 family in raw commingled cow milk above or below tolerance and/or safe levels.

【Principle】

The kit is a competitive colloidal gold immunoassay method. Sample is added to sample hole, if there is Aflatoxin M1 (AFM1-0.5) in samples, it will combine with marked antibodies, preventing the marked antibodies from combining the Aflatoxin M1 (AFM1-0.5) antigens of NC nitrocellulose membrane.

【Technique Data】

• Kit sensitivity: 0.5 ppb (ng/ml) (Final detection limit)

【Kit Contents

Test strip ………………………… 12*8T/kit

96 well tray and sample well with reagent.

Instruction …………………………..1

【Milk Sample Preparation】

1. Collect milk sample from butch, or farm

2. Make sure the milk has no clot.

3. Milk at room temperature, don’t heat or freeze.

4. Thoroughly mix with the reagent before testing.

【Test Procedure】

1. Read the manual instruction before testing. Be sure the raw commingled milk and test kit is at room temperature.

2. Take one Tube from the box, open tube and take out decided number of test to be perform, Use the test kit within an hour beyond time may cause failure of the test. The unused test strip must be sealed and keep out from sunlight and prevent moisture.

3. Take the milk sample, drop 200ul (about 4-5drops) into the sample-well, and thoroughly dissolve the milk with reagents for 5 minutes till the milk presents pink color.

4. Insert the test strip into the sample-well, read the results after 5 minutes.

【Results Judgment】

Negative(-): control line(C) display red, test line(T) color is deeper than the control line or the same as the C line, the result shows that (AFM1-0.5) concentration is less than detection Limits or not containing (AFM1-0.5).

Positive(+): control line(C) display red, test line(T) color is shallower than the control line or no color, the result shows that (AFM1-0.5) concentration is higher than detection Limits.

Invalid: control line (C) doesn’t display, it means the operational process is incorrect or this test card has lost efficacy.

【Notice】

• Don’t use the expired or damaged products
• The test card removed from refrigerator should place at room temperature and should be used as soon as possible to avoid failure when wet
• Don’t touch sample hole and white nitrocellulose (NC) membrane
• Don’t mix micropipette to avoid cross contamination
• Samples should be clear, not turbid particles and not clogged; if not, it will easily lead to blocking and inconspicuous color, which will affect the judgment of the experimental results.