Clenbuterol Hydrochloride Colloidal Gold Rapid Detection Card

Clenbuterol Hydrochloride Colloidal Gold Rapid Detection Card

operation instructions

(Colloidal gold method)

1. Principle and Application

This product is made by applying the principle of competitive inhibition colloidal gold immunochromatography to detect Clenbuterol hydrochloride (CLE) in urine, tissue, feed and other samples.

After the sample solution is dropped into the sample adding hole of the detection card, the Clenbuterol hydrochloride in the sample solution is combined with the gold labeled antibody, thus preventing the gold labeled antibody from combining with the Clenbuterol hydrochloride conjugate on the cellulose membrane. When the content of Clenbuterol hydrochloride in the sample solution is greater than the detection limit, the detection line does not show color, and the result is positive; When the content of Clenbuterol hydrochloride in the sample solution is less than the detection limit, the detection line will show purplish red, and the result is negative.

2. Technical indicators

2.1 Lower limit of sample detection:

Urine sample: 3 ppb

Organization: 3ppb

Feed: 30 ppb

3. Kit composition

Detection card: 40 pieces/box

Instructions: 1 copy

4. Required equipment and reagents

4.1 Instruments: homogenizer, nitrogen drying device, oscillator, centrifuge, graduated pipette, balance (sensitivity 0.01g)

4.2 Micro Pipette: single channel 20 µ l-200 µ l, 100 µ l-1000 µ l

4.3 Reagents: anhydrous sodium sulfate, n-hexane, methanol

5. Sample pretreatment

5.1 Instructions before sample processing:

The experimental equipment must be clean and use a disposable suction head to avoid contamination and interference with the experimental results.

5.2 Sample preprocessing steps:

5.2.1 Urine sample

Take a clear urine sample for supernatant testing. If the urine sample is cloudy, it needs to be centrifuged at 4000r/min for 10 minutes, and the supernatant should be taken for detection.

5.2.2 Organization

Weigh 3 ± 0.05g of homogeneous fresh muscle/liver tissue samples and place them in a 50ml covered centrifuge tube. Add 2 ml of deionized water and shake for 5 minutes before boiling for 5-10 minutes; Allow to stand for 5 minutes, and after cooling, take the supernatant for testing.

5.2.3 Feed

1) Weigh 1.0 ± 0.05g of the homogenized feed sample, add 1g of anhydrous sodium sulfate, and then add 10ml of methanol, shake for 3 minutes; Centrifuge at room temperature above 4000r/min for 10 minutes.

2) Suck out 1ml of the supernatant after centrifugation, dry it in air or nitrogen at 50-60 ℃, dissolve it in 1ml of deionized water, and then add 1ml of n-hexane to mix for 30 seconds; Centrifuge at room temperature above 4000r/min for 5 minutes.

3) Take 80ul of lower liquid for testing.

6. Experimental Steps

6.1 Remove the inspection card and place it on a flat and clean surface.

6.2 Use a matching pipette to absorb the prepared sample liquid, and slowly and dropwise add 2-3 drops (approximately 60ul)

into the sampling hole (S).

6.3 Leave at room temperature for 8-10 minutes to determine the results.

7. Result judgment

Negative: Both the C and T lines show color, indicating that the concentration in the sample is below the detection limit or does not contain.

Positive: The C line shows red, while the T line does not show color, indicating that the concentration in the sample is above the detection limit.

Invalid: The absence of a quality control C-line indicates an incorrect operating process or a failure of the detection card.

8. Precautions

8.1 Products that have expired or have damaged aluminum foil bags cannot be used.

8.2 When removing the detection card from the refrigerator, it should be restored to room temperature before opening. The opened detection card should be used as soon as possible to avoid failure due to moisture.

8.3 Do not touch the white film surface in the center of the detection card.

8.4 Droppers should not be mixed to avoid cross contamination.

8.5 The sample solution to be tested should be clear, free from turbid particles, and bacterial contamination, otherwise it may lead

to abnormal phenomena such as blockage and unclear color development, which may affect the judgment of experimental results.

9. Storage and shelf life

Storage conditions: The reagent kit should be stored in a dry environment at 2-30 ℃.

Shelf life: The product has a validity period of 1 year, and the production date can be found in the packaging box.