Chicken Infectious Bursal DiseaseAb Rapid Detection Card

Chicken Infectious Bursal DiseaseAb Rapid Detection Card

operation instructions

(Colloidal gold method)

【product name】

Common name:Chicken Infectious Bursal DiseaseAb Rapid Detection Card

【Packaging specifications】 40T/box

【Intended Use】 Infectious bursa of Fabricius (IBD), also known as Gamblo disease, is an acute and highly infectious disease caused by infectious bursa of Fabricius virus. Due to its sudden onset, short course, high mortality rate, and the ability to cause immune suppression in chickens, this disease remains one of the main infectious diseases in the chicken industry. Through the joint efforts of scientific research departments and a large number of veterinary workers, the disease has been effectively controlled to a certain extent.

This reagent is used to detect infectious bursa of Fabricius virus antibody in poultry serum, and can be used for immune effect evaluation, auxiliary diagnosis, etc.

【Principle】

This reagent is made based on the principle of competitive colloidal gold immunochromatography (GICA), and has good sensitivity to different subtypes of infectious bursa of Fabricius virus antibodies. After the sample is added to the sample well, it moves along the chromatographic membrane with IBD antigen and colloidal gold markers. If there is IBD antibody in the sample, it competes with the colloidal gold marker for IBD antigen, so that the detection line does not show purple red. If there is no IBD antibody in the sample, a color reaction occurs.

【Storage and expiration date】 Store in a cool and dry place (2-30 ℃), valid for 24 months.

【Sample preparation】

Serum: Extract 2-3ml of blood according to the conventional method and place it in a clean and dry test tube. Let it stand for about 1 hour until the blood clots, then centrifuge at 4000 rpm for 10 minutes (or let the blood stand for about 2 hours until the blood clots and naturally precipitates serum), and separate the serum. Require clear serum, no hemolysis, and no contamination. Serum samples can be stored at 2-8 ℃ for a short period of time, but need to be stored at -20 ℃ for a long period of time.

Whole blood: Whole blood with anticoagulants can be used as the test sample (whole blood samples cannot be frozen).

【test method】

1. Tear open the aluminum foil packaging bag of the inspection card, take out the inspection card, and place it on a flat and clean tabletop.

2. Use a matching pipette to suck the prepared sample, and vertically and slowly add 1 drop (about 25ul) into the sampling hole (at this time, there is no liquid flowing out of the observation hole).

3. Wait for 10 minutes and then add two drops (approximately 60ul) of buffer solution to the sampling hole using a dropper bottle filled with buffer solution.

4. Leave it at room temperature for 10-15 minutes to determine the results. If it exceeds 30 minutes, the results will be invalid.

Attention: Excessive addition of samples or buffer solution may result in incorrect results.

【Result judgment】

1. Negative: In the observation hole, both the detection line area (T) and the control line area (C) show a purple red line. This means that the antibody level is lower than the HI titer of 1:16.

2. Positive: Within the observation hole, only the control line area (C) shows a purple red line. It means that the antibody level is equal to or higher than the HI titer 1:32.

3. Invalid: No color lines appear in the control line area (C) within the observation hole.

The detection threshold of this reagent is HI test 1:32. So by performing multiple dilution on the tested serum and finding the maximum dilution ratio at which it appears positive, the antibody titer can be obtained. For example, if the serum to be tested is diluted with physiological saline at 1:2 (one part of the serum and one part of the physiological saline, and so on), 1:4, and 1:8, and then three samples with different dilution ratios are tested separately. If 1:2 and 1:4 are both positive, and 1:8 is negative, the maximum dilution ratio for positive results is 1:4, and the final antibody titer of this sample is 25 (1:32) multiplied by 22 (1:4), which is 27 (1:128).

【Interpretation of test results】

For the immunized samples, the level of antibodies reflects the level of immune efficacy. For samples that have not been immunized, positive results indicate possible IBD infection, and analysis should be conducted in conjunction with clinical and other methods.

【Limitations of Test Methods】

This test is only used to detect the level of IBD common antibodies and does not conduct subtype analysis.

【note】

1. Please read the instructions carefully before the experiment. The various reagents provided in this product are for the purpose of this experiment only.

2. Expired or damaged aluminum foil bags cannot be used.

3. When removing the detection card from the refrigerator, it should be restored to room temperature before opening. The opened detection card should be used as soon as possible to avoid failure due to moisture.

4. The samples to be tested should be fresh and clear, avoiding the use of contaminated, turbid, severely hemolytic, high blood lipids, and abnormally viscous samples.

5. Avoid touching the chromatographic membrane at the sample hole and detection window on the detection card.

6. The waste from the experiment should be considered as pollutants and properly disposed of in accordance with local regulations.